Fatima-Azzahra Delmani, Adnan S. Jaran, Mariam Abu-Al basal, Kaplan. N and Mai Al Abdallah (2024). Antimicrobial Resistance and Plasmid Profiles of Pseudomonas aeruginosa Isolated From Different Clinical Samples In Jordan. Malaysian Journal of Microbiology. Accepted.

Aim: The aims of this study were to investigate plasmid profiles and antibiotic resistance patterns of Multi drug resistant Pseudomonas aeruginosa strains isolated from different clinical specimens. Methodology: Pseudomonas aeruginosa strains were isolated from different clinical specimens from different hospitals and primary health care centers. The antimicrobial susceptibility of Pseudomonas aeruginosa isolates was determined using disc diffusion method against 16 commonly used antimicrobial drugs. Plasmid DNA was extracted from lysed Pseudomonas aeruginosa cells using Plasmid alkaline lysis method and visualized using Agarose gel electrophoresis and stained with ethidium bromide. Results: The results showed that isolated strains of Pseudomonas aeruginosa were resistant to Cefepime (90%), Meropenem (70%), Ceftazidime (60%), Piperacillin (55%), Aztreonam (50%), Ciprofloxacin and Tobramycin (35%), Gentamicin (29%), Imipenem and Amikacin (20%). And sensitive to Colistin. Plasmid analysis of clinical isolates showed the presence of 0 to 3 plasmids with size range of 1 to 25 Kbp. Compared to Standard strains of E.coli (ATCC 25922). Conclusion: The results obtained in this study showed some correlation between the patterns of antibiotic resistance and plasmid profiles.